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    Zymo Research — 16S rRNA sequencing laboratory

    Living Microbe Report

    Our most biodiverse ferment — the widest wild cast of the three.

    The Living Jar

    LactobacillusLeuconostocOther wild bacteria

    Share of bacterial DNA reads

    • Role in the ferment

      The engine. Acid-loving bacteria that sour and preserve the jar with no heat and no vinegar. Sequencing indicated Lactobacillus brevis and the cold-loving L. sakei — a signature of kimchi-style ferments — within this group.

      Flavor

      Clean lactic tang with a deeper, savory edge from L. sakei.

      Where it comes from

      Rides in on the raw vegetables, chiles, and aromatics.

      Why it matters

      Its acid keeps the ferment alive and stable; L. sakei keeps it working even at fridge temperatures.

    • Role in the ferment

      The widest long tail of minor bacteria we've sequenced — the natural diversity that the serrano, tomatillo, cilantro, and aromatics bring in.

      Flavor

      Collectively, layered background complexity.

      Where it comes from

      The natural microbial diversity of a produce-rich recipe.

      Why it matters

      This is biodiversity itself — the reason Mexicana reads as our most complex, alive ferment. As it matures and acidifies, this fraction shrinks.

    • Role in the ferment

      Starts fermentation in the first days and makes the CO₂ behind the gentle effervescence.

      Flavor

      Bright, tangy, lightly effervescent.

      Where it comes from

      Everywhere on fresh produce; the classic first-mover.

      Why it matters

      Drops the pH and hands off to the acid-loving finishers.

    How a raw ferment sours itself

    living-ferment zonepH 6.0pH 3.2Day 0JarredLeuconostoc kicks it offLactobacillus drives it homeSour, stable, alive —around pH 3.5

    Every batch follows this arc: as the bacteria feed, they make lactic acid and the pH falls, until it's sour enough to keep the jar alive and shelf-stable without heat or vinegar. Typical progression across our ferments.

    Sequenced by Zymo Research · 16S rRNA targeted sequencing (V3–V4) · September 2026. Genus identifications are reliable; species names are indicative. Percentages are share of bacterial DNA reads; plant DNA excluded. Types observed is Zymo's observed-species count and may include a few plant DNA signals.

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